ABSTRACT The purpose of this study is to validate the applicability of Technology Acceptance Model (TAM) in mobile banking perspective and explore the gender differences and m-banking self-efficacy as external variables. From the review of previous studies, it revealed that very few studies had developed research models statistically validate the role of gender on the intention of users to adopt mobile banking (m-banking). A total of 247 valid respondents in India were tested against the research model using analysis of variance and structural equation modeling (SEM) approaches. The results of current study recommend that the model was satisfactorily validated by the data. Overall, the model explained 42.8% of the variance in intention to use mbanking system. The findings have also favored gender as key determinants in the TAM. Based on these findings, the theoretical and practical implications are discussed. Keywords: Technology Acceptance Model (TAM); Gender differences; Mobile Banking (m-banking);mbanking self-efficacy.